Volume 34 Number 3 (2025)

High-Risk Human Papilloma Virus Genotyping in Patients with Clinically Suspected Precancerous and Cancerous Lesions of Cervix

Rashid JS1 , Rahman P2 , Khan RR3 , Afrin SS4 , Mudi N5 , Saha MK6 , Yeasmin F7 , Karim SS8 , Dewan RK9 , Zaman P10 , Rahman M11

Mymensingh Med J 2025 Jul; 34 (3): 816-824

PMID: 40583674

Abstract

Nowadays, human papilloma virus (HPV) testing has been added with cytology to aid in detection of high-grade cervical lesions, especially in women aged ≥30 years. This study aimed to evaluate the relationship between high-risk HPV (hrHPV) genotype and clinicopathological parameters among the diagnosed patients with both precancerous and cancerous cervical lesions. This cross-sectional study was carried out in the Department of Pathology of Dhaka Medical College and in the collaboration with ‘DNA Solution LTD, Dhaka’, Bangladesh. The study was conducted over a period of 24 months from July 2018 to June 2020. Following informed written consent, a total of 51 women (18 patients with clinically suspected malignant lesions and 33 with a positive VIA test) were included in this study, and all were subjected to colposcopic examination performed by a gynecologist. Ethical issues were ensured in accordance with the 1975 Declaration of Helsinki. SPSS version 24.0 was used during data analysis. The average age ±SD of the patients with cervical lesions was 41.86±9.84 years with a range from 25 to 60 years. Approximately half of the participants (n=25, 49.0%) were married for less than 16 years and almost one-tenth were multiparous. Among all cytological and histological findings, hrHPV-16 (n=21, 41.18%) was commonest, followed by hrHPV-18 (n=3, 5.88%) and hrHPV-68 (n=1, 1.96%). Two patients had co-infection with hrHPV (16, 18) and hrHPV (16, 51) in particular. However, the rest 24 cases remained undetected. Among the hrHPV-positive cases (n=27), hrHPV-16 was detected in 5 cases while those were negative for intraepithelial lesions.  In cervical swabs, 6 cases of hrHPV-16, 2 cases of hrHPV-18 and one case of co-infection hrHPV (16, 18) were histologically diagnosed as invasive SCC G-II. Among 51 cases, the most common histological diagnosis was CIN I (n=18, 35.3%). hrHPV genotyping was found to be associated with cytological findings and histological diagnosis.

Keywords: Cervical cancer, Ca cervix, HPV, Human papilloma virus, CIN, ASCUS, VIA


  1. Assistant Professor

    Department of Pathology, Sir Salimullah Medical College, Dhaka, Bangladesh

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Volume 34, Number 3 (2025)
Page: 816-824