Volume 34 Number 2 (2025)
Antibacterial Activities of Mint (Mentha piperita) Leaf Extracts (Aqueous) Against Gram Positive Staphylococcus aureus and Gram Negative Pseudomonas aeruginosa
Afrin A1 , Saha SK2 , Zannat KE3 , Hossain MA4 , Saha BC5 , Joynal JB6
Mymensingh Med J 2025 Apr; 34 (2): 355-359
Abstract
The interventional study was undertaken to evaluate the antibacterial activity of aquous extracts isolated from Mint (Mentha piperita) leaf against Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. This study was performed in the Department of Pharmacology and Therapeutics in collaboration with the Department of Microbiology at Mymensingh Medical College, Mymensingh, Bangladesh from January 2021 to December 2021. The antibacterial effect of different concentrations of aqueous Mint leaf extracts was tested by using disc diffusion and broth dilution method. The test microorganisms underwent a broth dilution procedure to assess their activity against the standard antibiotic Gentamicin. The outcome of this test was compared to that of aqueous extracts. Among different concentrations of the aquous mint leaf extract (AMLE), 200µg/ml and above concentration showed inhibitory effect against Staphylococcus aureus and 600µg/ml and above concentration showed inhibitory effect against Pseudomonas aeruginosa. The MIC of Gentamicin was much lower in comparison to MICs of AMLE for the test organisms. This in vitro study suggests that aqueous extract of Mint leaves possess antibacterial effect against Staphylococcus aureus and Pseudomonas aeruginosa.
Keywords: Antibacterial effects, Mint, Staphylococcus aureus, Pseudomonas aeruginosa, Zone of inhibition, Minimum inhibitory concentration, Disc diffusion and broth dilution method, Aqueous extract
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